Search results for "Single shot"
showing 9 items of 9 documents
Optical sectioning microscopy through single-shot Lightfield protocol
2020
Optical sectioning microscopy is usually performed by means of a scanning, multi-shot procedure in combination with non-uniform illumination. In this paper, we change the paradigm and report a method that is based in the light field concept, and that provides optical sectioning for 3D microscopy images after a single-shot capture. To do this we fi rst capture multiple orthographic perspectives of the sample by means of Fourier-domain integral microscopy (FiMic). The second stage of our protocol is the application of a novel refocusing algorithm that is able to produce optical sectioning in real time, and with no resolution worsening, in the case of sparse f luorescent samples.We provide the…
The Lightfield Microscope Eyepiece
2021
Lightfield microscopy has raised growing interest in the last few years. Its ability to get three-dimensional information about the sample in a single shot makes it suitable for many applications in which time resolution is fundamental. In this paper we present a novel device, which is capable of converting any conventional microscope into a lightfield microscope. Based on the Fourier integral microscope concept, we designed the lightfield microscope eyepiece. This is coupled to the eyepiece port, to let the user exploit all the host microscope’s components (objective turret, illumination systems, translation stage, etc.) and get a 3D reconstruction of the sample. After the optical design, …
Depth rendering of large incoherent scenes from integral images
2016
Integral imaging is a technique that provides the spatial and angular information of three-dimensional (3D) scenes through a single shot. Taking advantage of this capability, different applications have been developed. Some of these applications are the 3D display and digital post-processing, in particular depth-reconstruction from integral images.
Mathematical models for the diffusion magnetic resonance signal abnormality in patients with prion diseases
2014
In clinical practice signal hyperintensity in the cortex and/or in the striatum on magnetic resonance (MR) diffusion-weighted images (DWIs) is a marker of sporadic Creutzfeldt–Jakob Disease (sCJD). MR diagnostic accuracy is greater than 90%, but the biophysical mechanisms underpinning the signal abnormality are unknown. The aim of this prospective study is to combine an advanced DWI protocol with new mathematical models of the microstructural changes occurring in prion disease patients to investigate the cause of MR signal alterations. This underpins the later development of more sensitive and specific image-based biomarkers. DWI data with a wide a range of echo times and diffusion weightin…
Robust quantum control by a single-shot shaped pulse
2013
Considering the problem of the control of a two-state quantum system by an external field, we establish a general and versatile method allowing the derivation of smooth pulses which feature the properties of high fidelity, robustness, and low area. Such shaped pulses can be interpreted as a single-shot generalization of the composite pulse-sequence technique with a time-dependent phase.
Single-shot generation and detection of a two-photon generalized binomial state in a cavity
2006
A "quasi-deterministic" scheme to generate a two-photon generalized binomial state in a single-mode high-Q cavity is proposed. We also suggest a single-shot scheme to measure the generated state based on a probe two-level atom that "reads" the cavity field. The possibility of implementing the schemes is discussed.
Lightfield microscopy, an emerging tool for real-time 3D imaging
2020
Integral, or lightfield, microscopy offers the possibility of capturing and processing in real time multiple views of 3D fluorescent samples captured with a single shot. In this contribution we review the recent advances in lightfield microscopy and enunciate the forthcoming challenges.
Alignement moléculaire par impulsions laser ultrabrèves : Mesures & Applications
2009
The interaction of an intense ultrashort laser pulse with a molecular sample leads, after the end of the pulse, to molecular alignment at specific time under field-free conditions. This thesis is dedicated to the measurements and applications of this effect. The degree of alignment is determined through the variation of the refractive index induced by the rearrangement of the molecular dipoles. A two-dimensional technique allowing to measure molecular alignment on a single-shot basis is reported in this document. The alignment is then exploited in order to determine physical quantities which are usually difficult to estimate through others methods. The "cross-defocusing technique" has been …